triptolide treatment group  (MedChemExpress)

Journal: IBRO Neuroscience Reports

Article Title: Neuroprotective effect of triptolide on neuronal inflammation in rats with mild brain injury

doi: 10.1016/j.ibneur.2024.05.007

Figure Lengend Snippet: Pathological changes in the hippocampus of mTBI rat. A.HE staining showed that the swelling and rupture of rat cells were decreased after triptolide treatment. B. number of neuron cells. Swelling and ruptured nerve cells can be seen in the image, as shown by the black arrow. ## P < 0.01 mTBI compared with the sham group in both day 3 and day 7; *P < 0.05, mTBI+TP compared with the mTBI group day 3 and day 7. scale bar 100 µm, n=3. C. NeuN immunostaning (red). D. NeuN positive cells.comparison between groups on day 7, and day 3 each group. ## P < 0.01 mTBI compared with the sham group in both day 3 and day 7; *P < 0.05, mTBI+TP compared with the mTBI group day 3 and day 7. Data presented as mean + SD. n=5. The *,and # , indicate statistically significant differences.

Article Snippet: The rats in the triptolide treatment group were intraperitoneally injected with 0.2 mg/kg triptolide ( ) for a week.TP (Med Chem Express, USA#39,836) was dissolved in DMSO at a concentration of 2 mg/mL and diluted with normal saline to a final concentration of 0.2 mg/ mL.

Techniques: Staining, Comparison

Journal: IBRO Neuroscience Reports

Article Title: Neuroprotective effect of triptolide on neuronal inflammation in rats with mild brain injury

doi: 10.1016/j.ibneur.2024.05.007

Figure Lengend Snippet: Inflammatory-related factors A. IL-1β, B. TNF-α and C. IL-10; changes after triptolide treatment. D&E individual raw data of each factors after 3 days and 7 days. Comparison between groups on day 3, comparison between groups on day 7, and day 3 and day 7 for each group. Data presented as mean + SD. The *, # , indicate statistically significant differences. IL-1β; mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01 , mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 # P < 0.05, TNF-ɑ; mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01, mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 ## P < 0.01, IL-10; mTBI Vs. Sham day3 * P < 0.05 , day 7 * P < 0.05, mTBI+TP Vs . mTBI; day3 ** P < 0.01, day 7 ** P < 0.01, n=10.

Article Snippet: The rats in the triptolide treatment group were intraperitoneally injected with 0.2 mg/kg triptolide ( ) for a week.TP (Med Chem Express, USA#39,836) was dissolved in DMSO at a concentration of 2 mg/mL and diluted with normal saline to a final concentration of 0.2 mg/ mL.

Techniques: Comparison

Journal: IBRO Neuroscience Reports

Article Title: Neuroprotective effect of triptolide on neuronal inflammation in rats with mild brain injury

doi: 10.1016/j.ibneur.2024.05.007

Figure Lengend Snippet: Triptolide downregulated IL-1β and NF-κB expressions. The relative mRNA expression was calculated using the formula 2– ΔΔ Ct. Comparison between groups on day 3, comparison between groups on day 7, and day 3 and day 7 for each group. Data presented as mean + SD. The indicate statistically significant differences. IL-1β; mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01 , mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 # P < 0.05, NF-kB; mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01, mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 ## P < 0.01, n=8.

Article Snippet: The rats in the triptolide treatment group were intraperitoneally injected with 0.2 mg/kg triptolide ( ) for a week.TP (Med Chem Express, USA#39,836) was dissolved in DMSO at a concentration of 2 mg/mL and diluted with normal saline to a final concentration of 0.2 mg/ mL.

Techniques: Expressing, Comparison

Journal: IBRO Neuroscience Reports

Article Title: Neuroprotective effect of triptolide on neuronal inflammation in rats with mild brain injury

doi: 10.1016/j.ibneur.2024.05.007

Figure Lengend Snippet: LC3B expression changes after triptolide treatment at different time points. (A): Representative LC3B positive (green) and DAPI (blue) merged image is shown. In the mTBI group, the positive expression index of autophagy protein LC3B was higher than both sham and mTBI + TP groups. The autophagy protein experimental group can be seen in the image. Scale bar = 50 μm. Immunofluorescence showed decreased expression of autophagy and aquaporin in rats treated with triptolide. (B): Comparison between groups on day 3, comparison between groups on day 7, and day 3 and day 7 for each group. Data presented as mean + SD. The *, #, indicate statistically significant differences. mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01 , mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 ## P < 0.01 day 7, n=8. C. LC3B Western blot, D. Quantitative analysis of LC3B; mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01, mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 ## P < 0.01 day 7, n=3.

Article Snippet: The rats in the triptolide treatment group were intraperitoneally injected with 0.2 mg/kg triptolide ( ) for a week.TP (Med Chem Express, USA#39,836) was dissolved in DMSO at a concentration of 2 mg/mL and diluted with normal saline to a final concentration of 0.2 mg/ mL.

Techniques: Expressing, Immunofluorescence, Comparison, Western Blot

Journal: IBRO Neuroscience Reports

Article Title: Neuroprotective effect of triptolide on neuronal inflammation in rats with mild brain injury

doi: 10.1016/j.ibneur.2024.05.007

Figure Lengend Snippet: AQP4 expression changes after triptolide treatment at different time points. (A): Representative AQP4 positive (green) and DAPI (blue) merged image is shown. In the mTBI group, the positive expression index of AQP4 was higher than the sham and mTBI + TP groups. The Aquaporin of an experimental group can be seen in the image. Scale bar = 50 μm. Immunofluorescence showed decreased expression of autophagy and aquaporin in rats treated with triptolide. (B): Comparison between groups on day 3, comparison between groups on day 7, and day 3 and day 7 for each group. Data presented as mean + SD, n=8. The *, #, indicate statistically significant differences. mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01 , mTBI+TP Vs. mTBI; day3 # P < 0.05, day 7 # P < 0.05 day 7, n=8. C. LC3B Western blot, D. Quantitative analysis of LC3B; mTBI Vs. Sham day3 ** P < 0.01, day 7 ** P < 0.01, mTBI+TP Vs. mTBI; day3 ## P < 0.01, day 7 ## P < 0.01 day 7, n=3.

Article Snippet: The rats in the triptolide treatment group were intraperitoneally injected with 0.2 mg/kg triptolide ( ) for a week.TP (Med Chem Express, USA#39,836) was dissolved in DMSO at a concentration of 2 mg/mL and diluted with normal saline to a final concentration of 0.2 mg/ mL.

Techniques: Expressing, Immunofluorescence, Comparison, Western Blot